Class B trichothecene chemotyping in Fusarium species by PCR assay
Yörük E., Gazdağli A., Albayrak G.
Citation
Yörük E., Gazdağli A., Albayrak G. (2014). Class B trichothecene chemotyping in Fusarium species by PCR assay. Genetika 46(3): 661-669. https://doi.org/10.2298/gensr1403661y
Abstract
Fusarium isolates are divided into three chemotypes according to produce of
class B trichothecenes; 3-acetyldeoxynivalenol (3ADON)
15-acetyldeoxynivalenol (15ADON) and nivalenol (NIV) and 4-
acetyldeoxynivalenol (NIV) chemotypes. In this study, chemotyping of
seventeen isolates from Turkey and Iran belonging to F. graminearum, F.
culmorum, F. poae and F. pseudograminearum species were carried out by
polymerase chain reaction (PCR) analysis. While all F. culmorum and F. poae
isolates determined as 3ADON, remaining F. graminearum and F.
pseudograminearum isolates were either 3ADON or 15ADON chemotypes. A common
band of 583 bp long DNA fragment was amplified in all of F. culmorum and F.
poae, one F. pseudograminearum (21F) and four F. graminearum (14F, sh14,
sh15, sh7) isolates with 3ADON chemotype. However, remaining two F.
pseudograminearum and four F. graminearum isolates with 15ADON chemotype,
yielded amplicons that of 863 bp. It was shown that 3ADON was more
predominant chemotype from other class B trichothecenes. This is the first
report on chemotyping of F. poae and F. pseudograminearum isolates and also
to show presence of 3ADON chemotype in F. graminearum isolate from Turkey.